microbe Enterobacter Aerogenes. I performed multiple biochemical test to come to the conclusion of my specific microbe. Please see below for my microbe results and attached rubric for paper.
Paper Criteria (please see attached for more details)
-Sugar Fermentation (Lactose, Dextrose, and Sucrose)- positive for both acid and gas production.(Hence “AG” via chart attached)
-Triple suagr iron (TSI)- positive for acid production
-Methyl Red Voges Proskauer (MVRP) Test- MR Reaction was (-) no glucose breakdown /no color change, VP Reaction was (+) glucose breakdown to acetylmethylcarbinol – red ring around top of broth
-Citrate Utilization Test- (+) citrate utilization giving alkaline PH- royal blue media
-Sulfide, Indole, & Motility (SIM Test)- Sulfide- (-) no H2S to FeS/ no color change,Indole (+) indole production cherry red at top of agar, Motility was (+) due to cloudiness throughout agar
-Gelatin (Protein) Hydrolysis- (-) gelatinase was produced medium remains liquid after refrigeration.
-Starch Hydrolysis – (-) no starch hydrolysis agar is blue/blackincluding around bacterial growth
-Casein (Milk) Hydrolysis- (+) casein hydrolyzed clear zone around bacterial growth.
-Lipid Hydrolysis- (-) no lipid , opaque agar
-Urea Hydrolysis- (-) no urea/color change
-Nitrate Reduction- (+) reduced nitrates to nitric oxide, nitrous oxide or nitrogen gas, no color change
-Oxidase Test- (-) no cytochrome oxidase activity/no color change
-Catalase Test- (+) catalase production, bubbles appear
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